TNFR2( TNFR1 KO) Effector Reporter Cell
CBP74539
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| I. Background | |
| TNFR2（腫瘤壞死因子受體2）是TNFα的特異性受體之一，屬于TNFR超家族，其主要通過激活NF-κB等信號通路介導(dǎo)細(xì)胞存活、增殖和免疫調(diào)節(jié)功能，是自身免疫性疾病及腫瘤免疫治療等領(lǐng)域的重要靶點(diǎn)。 | |
| II. Description | |
| TNFR2(TNFR1 KO) Effector Reporter Cell? 是一個(gè)經(jīng)過基因工程改造的、用于特異性檢測TNFα-TNFR2信號通路活性的報(bào)告細(xì)胞系。其核心設(shè)計(jì)是：在效應(yīng)細(xì)胞中敲除TNFR1基因，并引入對TNFR2信號響應(yīng)的報(bào)告基因，從而提供一個(gè)純凈、高靈敏度的平臺，用于研究TNFα通過TNFR2（而非TNFR1）介導(dǎo)的生物學(xué)效應(yīng)、藥物篩選及抗體功能評價(jià)。 | |
| III. Introduction | |
| Expressed gene: | TNFR2 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+800 μg/ml Hygromycin B+1 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
 Figure 1. Sanger of TNFR2( TNFR1 KO) Effector Reporter Cell.  Figure 2. Dose Response of TNFR2 Agonist Ab in TNFR2(TNFR1 KO) Effector Reporter Cell(C64).  Figure 3. Dose Response of TNFR2 Agonist Ab in TNFR2(TNFR1 KO) Effector Reporter Cell(C64).  Figure 4. Blocking of TNFα induced TNFR2( TNFR1 KO) Effector Reporter Cell(C64) Activity by TNFα Neutralization Abs with Membrane Anchored TNFα Cell(Adherent, C12).  Figure 5. Blocking of TNFα induced TNFR2(TNFR1 KO) Effector Reporter Cell( C64) Activity by TNFR2 Blocking Ab with Membrane Anchored TNFα Cell ( Adherent, C12). |
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