c-Kit(GNNK-) CHO
CBP74541
詢 價(jià)
索取Protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫(kù)
| I. Background | |
| c-Kit(GNNK-)是c-Kit受體酪氨酸激酶的一種剪接變體，其胞外近膜區(qū)缺少GNNK（甘氨酸-天冬酰胺-天冬酰胺-賴氨酸）四肽序列，這種結(jié)構(gòu)差異可能影響其與配體結(jié)合后的二聚化及下游信號(hào)傳導(dǎo)特性。 | |
| II. Description | |
| c-Kit(GNNK-) CHO細(xì)胞，在 CHO-K1 細(xì)胞的基礎(chǔ)上，穩(wěn)定表達(dá) c-Kit(GNNK-) 。 | |
| III. Introduction | |
| Expressed gene: | c-Kit(GNNK-) |
| Host Cell: | CHO-K1 |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+ 10% FBS + 5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Binding Assay,FACS |
| IV. Representative Data | |
 Figure 1. Recombinant c-Kit(GNNK-) CHO constitutively expressing c-Kit(GNNK-) . |
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